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NEW VALIDATED HPLC METHOD FOR THE ESTIMATION OF METHYL HYDROXY BENZOATE IN TABLET FORMULATION

Author(s): V. Sreeram, V. D. N. Kumar Abbaraju, M. V. Basaveswara Rao and M. Sivanadh

ABSTRACT

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Methyl Hydroxy Benzoate in Bulk and Pharmaceutical tablet Formulation. Isocratic elution at a flow rate of 1.0 ml/min was employed on symmetry Luna C18 5μm (4.6 x 150 mm) or equivalent at ambient temperature. The mobile phase consisted of Methanol: Water in the ratio of 50:50 v/v. The UV detection wavelength was 254nm and 20µl sample was injected. The retention time for Methyl Hydroxy Benzoate is identified that 4.8 min. The Average percentage recovery of the method was in the range of 96.1 – 102.6%.The method was validated as per the ICH guidelines. The method was successfully applied for routine quality control analysis of pharmaceutical formulation.

Key Words: Methyl Hydroxy Benzoate, HPLC, UV detection, Recovery, Precise.

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